Eleven different species of yeasts isolated from raw milk, such as issatchenkia orientalis, pichia fermentans, rhodotorula mucilaginosa and yarrowia lipolytica were identified using an integrated approach, including phenotypic and genotypic methods and the api 20c aux system. We compared the auxacolor yeast identification system sanofi diagnostics pasteur with the api 20c aux. In all, 116 clinical isolates, 68 candida albicans, 12 c. Material api 20c aux strip incubation tray c medium. Two hundred twentyfive yeasts, yeastlike fungi, and algae, comprising 28 species. A total of 206 isolates belonging to 25 species was used in this study. To compare the performance of seven commercial yeast identification methods with that of a reference method, and to compare the costs of the commercial kits. The american petroleum institute api has released its publications programs and services catalog for 2016. Anaerobe laboratory manual 4th edition 1977 virginia polytechnic institute and state. Yeast identification by sequencing, biochemical kits. A corrigendum on comparison of 21plex pcr and api 20c aux, malditof ms, and rdna sequencing for a wide range of clinically isolated yeast species. Dalmau method, and profile number on api 20c aux biomerieux. Faster results were obtained in most cases with auxacolor.
Api has a wide identification database on the market and a numerical identification calculation method that brings standardization and makes identification easy and accurate. Ref 20 100 20 160 07584d gb 200210 20 e identification system for enterobacteriaceae and other nonfastidious gramnegative rods summary and explanation api 20 e is a standardized identification system for enterobacteriaceae and other nonfastidious, gramnegative rods which uses 21 miniaturized biochemical tests and a database. The wellestablished method for manual microorganism identification to the species level, biomerieuxs api identification products are test kits for identification of gram positive and gram negative bacteria and yeast. Dupont examined 227 yeast strains with api 20 c aux and auxacolor. The performance of api 20c aux was higher with 87% 101 of 116 isolates than that of candifast with 82. Api 20c facilitated correct identification of 97% common and 88% rare isolates while id 32c facilitated correct identification of 92% common and 85% rare isolates. Dec 01, 2014 other api system api anaerobe identification api 20a 24hour identification of anaerobes rapid id 32a 4hour identification of anaerobes api yeast identification api 20c aux 48 to 72hour identification of yeasts others api 50 ch performance of carbohydrate metabolism tests api zym semiquantitation. A catalog of api publications and materials is published annually and updated quarterly by api, 1220 l street, n. Frontiers comparison of 21plex pcr and api 20c aux, maldi. Identification of the causative organism and the disease extension are the first steps in the management of the affected patients and predicting disease treatment outcome and prognosis. Microbiologics api 20c aux comprehensive qc set kwikstik. Api spec 20a carbon steel, alloy steel, stainless steel, and nickel base alloy castings for use in the petroleum and natural gas industry, second edition, includes addendum 1 2018 and addendum 2 2020 standard by american petroleum institute, 08012017.
Mycetoma is a unique neglected tropical disease caused by a substantial number of microorganisms of fungal or bacterial origins. Efficacy of api 20c and id 32c systems for identification of. Eleven selected strains which tolerated low ph levels, gastric juice. If identification was inconclusive less than 90% confidence on api, the antifungal susceptibility results for fluconazole and the api number generated were taken into account. Pdf comparison of 21plex pcr and api 20c aux, malditof. After 3 days, correct identification rates were found of 94. Oct 09, 2015 api 20e kit contains api 20e strip media reagents api strip trays api code chart api score sheet api webapi catalogue 10. About us we believe everything in the internet must be free. The supplies efficiently check food irradiation using accepted goldstandard food microbiology testing methods. Microbiologics api 20c aux comprehensive qc set microorganism sets for culture purposes and qc applications. With api 20 c aux, the correct identification rate was 89.
All yeast identification procedures were conducted in accordance with the manufacturers instructions. Using the api 20c system, we were able to distinguish most species of phialophora and cladosporium and to separate l. The fungichrom i method was found to be rapid, as 90% of strains were identified after incubation for 24 h at 30c. Api 20c aux, 21plex pcr, malditof ms, lsu rdna sequencing, developing countries citation. Improved identification by combining 21plex pcr and api 20c aux as an alternative strategy for developing countries. Clinical yeast isolates n 52, comprising 19 species, were identified using vitek, api id 32c, api 20c aux, yeast star, auxacolor, rapid yeast plus system, and api candida and compared with a reference method which. Api products have led to the identification of new bacterial species e. The performance of the rapid yeast plus system innovative diagnostic systems, norcross, ga. The api, id 32 and rapid id 32 database update takes into account. A total of 168 isolates were analyzed and the results were compared to those of the api 20c aux 24% o api id 32c 76% kits biomerieux, marcy letoile, france. Pdf efficacy of api 20c and id 32c systems for identification of. To compare the performance of current chromogenic yeast identification methods, three commercial systems api 20c aux, fungichrom i and candifast were evaluated in parallel, along with conventional tests to identify yeasts commonly isolated in this clinical microbiology laboratory. Efficacy of api 20c and id 32c systems for identification. The abilities of the api 20c and id 32c yeast identification systems to identify 123 common and 120 rare clinical yeast isolates were compared.
The catalog is a comprehensive resource for all api standards and practices, technical documents, and equipment specifications. Yeast identification by sequencing, biochemical kits, maldi. Different laboratorybased diagnostic tools and techniques were developed over. One of 14 strains of candida tropicalis and 10 of 16 strains of trichosporon cutaneum were not correctly identified. Bsl3 adds technical, quality and qualification criteria to bsl2. Conventional yeast identification methods were used as a reference. Pdf the abilities of the api 20c and id 32c yeast identification systems to identify 123 common and 120 rare. Top 4 feb 2019 api 20 c aux is a system for the precise identification of.
Recommended practice for analysis, design, installation, and testing of basic surface safety systems for offshore production platforms name of standards organization. Species identifiable by the various identification systems api 20 e gramnegative bacilli api 10 s gramnegative bacilli rapid 20etm enterobacteriaceae api 20 ne gramnegative nonenterobacteriaceae api staph staphylococci api 20 strep streptococci api candida yeasts api 20 c aux yeasts api 20 a anaerobes api coryne corynebacteria api campy campylobacter. Comparison of the api candida system with the auxacolor. With auxacolor, the percentages of correct identification after 1 and 2 days. Two hundred twentyfive yeasts, yeastlike fungi, and algae, comprising 28 species and. This item has been discontinued by the manufacturer and is no longer available. Eleven selected strains which tolerated low ph levels, gastric juice and bile salt were further analysed for the. Biomic v3 microbiology system automated api reader. A catalog of api publications and materials is published annually by api, 1220 l street, nw, washington, dc 20005. This specification does not apply to field use or field.
Bsl2 and bsl3 are intended to be comparable to bsl2 and bsl3 as found in api 20e. Clinical yeast isolates n 52, comprising 19 species, were identified using vitek, api id 32c, api 20c aux, yeast star, auxacolor, rapid yeast plus system, and api candida and compared with a reference method. The catalog also includes reports and studies regarding the safety, efficiency, and responsibilities of the petroleum industry. Repair and remanufacture of 16a equipment is now covered in api 16ar, standard for repair and remanufacturing of drillthrough equipment. Comparison of auxacolor with api 20 c aux in yeast identification. Within the food industry, use these tests to isolate and identify indicator organisms or foodborne pathogens. Evaluation of the auxacolor system for biochemical identification of. Api 20 microbial identification kits, biomerieux vwr. Comparison of 21plex pcr and api 20c aux, malditof ms, and rdna sequencing for a wide range. Test for the presence of microorganisms in media using the testing kits available. Comparison of auxacolor with api 20 c aux in yeast. Considering that 10 api strips are used every minute, apiweb is an invaluable tool for the microbiology community. Review of proficiency testing services for clinical laboratories in. Apilisteria 10300 listeria 2 ml api suspension medium 1 mcf na api gp 1824h 37c api20 c aux 20210 yeasts 2 ml api naci 0.
After inoculation and incubation, the reactions are interpreted by comparison to growth controls and use of the identification table provided with each kit. Comparative performance of fungichrom i, candifast and api. Pdf comparison of 21plex pcr and api 20c aux, malditof ms. Analytical profile index or using the identification software. Clinical spectrum and factors impacting outcome of candida. Frontiers comparison of 21plex pcr and api 20c aux. Standardized, miniature version of conventional methods conducting various microbial tests. Comparative performance of the rapid yeast plus system and. Clinical yeast isolates n 52, comprising 19 species, were identified using vitek, api id 32c, api 20c aux, yeast star, auxacolor, rapid yeast plus system, and api. By order of the executive director office of the federal register washington, d. Each isolate was grown in chromogenic agar and in corn meal agar oxoid, uk to observe its micromorphology. This specification covers the following product forms, processes, and sizes.
Specification for shop welded tanks for storage of production liquids. To compare auxacolor with api 20 c aux for identification of yeasts. The system offers a large and robust database now accessible through the internetbased apiweb service. Evaluation of albicans id and biggy agar for the isolation. The wellestablished method for manual microorganism identification to the species level, biomerieuxs api identification products are test kits for identification of. Api spec 20a carbon steel, alloy steel, stainless steel, and nickel base alloy castings for use in the petroleum and natural gas industry, second edition, includes addendum 1 2018 and addendum 2 2020 standard by american petroleum institute, 08012017 amendments available. A tool that leverages the technologies of today and tomorrow, apiweb is the essential complement to manual identification. The api 20c aux strip consists of 20 microtubes containing dehydrated substrates in which 19 assimilation tests are performed. Comparison of 21plex pcr and api 20c aux, malditof ms, and rdna sequencing for a wide range of clinically isolated yeast species. Carbohydrate assimilation is studied with the api 20c aux. Apiweb clinical diagnostics products biomerieux clinical.
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